Genómica: Laboratory of Genómica
Genomics-Flow Cytometry Unit is one of central services of Technology Support Center (CAT) at the Rey Juan Carlos University. It provides technological support in the fields of Genomics and Flow Citometry to research groups both from the own University and external users from other research centers. Access to the Unit services request is through the informatics tool Servilims that can be found on the CAT website.
Genómica: Members
Dña. Lucía Torres Carrillo
Davinia Hernández Córdoba
Genómica: Equipment
Secuenciador de DNA ABI 3130
DNA sequencing, Genotyping, mutation detection through DNA fragment analysis amplified by PCR and fluorescence labelled. Epigenetic studies by bisulfite treated-DNA sequencing.
Equipo de PCR en Tiempo Real (AB 7500 Fast)
Gene expression studies, DNA absolute quantification (microorganisms quantification in biological samples, Gene dosage quantification) and Genptyping (one nucleotide-polymorphism detection).
Equipo de PCR en Tiempo Real (AB 7000 SDS)
Gene expression studies, DNA absolute quantification (microorganisms quantification in biological samples, Gene dosage quantification) and Genptyping (one nucleotide-polymorphism detection).
Citómetro de flujo Beckman Coulter CytomicsFC 500MPL
Qualitative and quantitative determinations of biological and physical properties of cells when they pass one by one through a laser beam. 2 Laser beams and 5 fluorescence parameters.
Sorter modelo FACS Melody de Becton Dickinson
Analysis and physical separation of cells by flow cytometry. It allows depositing cells (even single cell) in tube or plate. 3 lasers and 8 fluorescence parameters
Escáner de fluorescencia Typhoon 9210
Fluorescence scanning, quantification and analysis from gels, membranes, slides and microplates. It allows simultaneous detection of 4 colours.
Sistema de fotodocumentación Chemidoc de Bio-Rad
Equipment for fluorescence and chemiluminescence imaging in gels and membranes and subsequent analysis of these images
Fluorímetro FluorStar Omega de Isogen
El FLUOstar® Omega es un lector de microplacas con seis modos de detección: intensidad de fluorescencia (incluyendo FRET), luminiscencia (incluyendo BRET), absorbancia UV/Vis, fluorescencia en tiempo resuelto (incluyendo TR-FRET), y AlphaScreen®/AlphaLISA®. Utiliza un espectrómetro ultravioleta ultravioleta / vis o filtros para la absorbancia, así como filtros altamente sensibles para todos los demás modos de detección.
Espectrofotómetro Nanodrop 1000
Spectrophotometer that allows working with very small sample volumes, up to 1.5 microliters. Determination of the concentration of DNA, RNA and proteins. It allows the detection of contaminants in the sample.
Fluorímetro Qbit4+Egel Power Snap System
Equipment to determine precise concentration and integrity of RNA, DNA and proteins by fluorometric reactions and highly sensitive gel electrophoresis
Genómica: Links
Genómica: Publications from the last 5 years
Publicaciones más relevantes en las que se ha hecho uso de los servicios de la Unidad de Genómica-Citometría:
1. Authors: CM Molina, N González, F. Bautista, R. Sanz, R Simarro, J. L. Sanz, i. Sanz
Title: Isolation and genetic identification of PAH degrading bacterium from a microbial consortium. Biodegradation 20, 789-800, 2009 SPRINGER
2. Chocarro-Calvo et al. La expresión del péptido insulinotrópico dependiente de glucosa (GIP) se induce sinérgicamente por insulina y glucosa en células entero endocrinas.Endocrinología y Nutrición, 2009. Mayo.: 56(especial congreso): 7
3. García-Martínez J.Met al. Transcriptional regulation of glucose dependent insulinotrópico peptide.Endocrinología & Metabología, 2009. 52, Suplemento 6 (II): 926
4. García-Redondo AB, et al. Losartan and tempol treatments normalize the increased response to hydrogenperoxide in resistance arteries from hypertensive rats. J Hypertens. 2009 Sep;27(9):1814-22.
5. Briones AM, et al. Atorvastatin prevents angiotensin II-induced vascular remodeling and oxidative stress. Hypertension. 2009 Jul;54(1):142-9.
6. Beltrán AE, et al. p38 MAPK contributes to angiotensin II-induced COX-2 expression in aortic fibroblasts from normotensive and hypertensive rats. J Hypertens. 2009 Jan;27(1):142-54.
7. Briones AM, et al. Activation of BK. Ca channels by nitric oxide prevents coronary artery endothelial dysfunction in ouabain-induced hypertensive rats. J Hypertens. 2009 Jan;27(1):83-91.
8. García-Redondo AB, et al. Hypertension increases contractile responses to hydrogen peroxide in resistance arteries through increased thromboxane A2, Ca2+, and superoxide anion levels. J Pharmacol Exp Ther. 2009, Jan;328(1):19-27.
9. Carrascosa JM, et al. Changes in the neuroendocrine control of energy homeostasis by adiposity signals during aging. Exp Gerontol: 44(1-2): 20-25 (2009)
10. Bonzón-Kulichenko E, et al. Central leptin regulates total ceramide content and srebp-1c proteolytic maturation in rat white adipose tissue. Endocrinology 150(1): 169-178 (2009)
11. García-Martínez, JM., et al. 2009. WNT/beta-catenin increases the production of incretins by entero-endocrine cells. Diabetologia 2009. 52:1913-1924. DOI 10.1007/s00125-009-1429-1.
12. Wagemakers, et al.Entraining synthetic genetic oscillators: Chaos 19 (2009): 033139
13. González-Rodríguez et al. Inhibition of PTP1B restores IRS1-mediated hepatic insulin signaling in IRS2-deficient mice. Diabetes. 2010 Mar;59(3):588-99. Epub 2009 Dec 22