INFRASTRUCTURE AND SERVICES
REY JUAN CARLOS UNIVERSITY
The “Support Center” of the Rey Juan Carlos University (URJC) is an administrative and management unit which depends on the Vice-Rectorate responsible for research. Its main purpose is to provide scientific and technological support to the research and practical training needs of the URJC and, in second instance, of its socioeconomic environment.
-GENOMICS (see Laboratory 170)
-FLOW CYTOMETRY:
Beckman Coulter Cytomycs FC 500MPL. It allows the sequencing or analysis of 4 samples in each electrophoresis and the simultaneous reading of up to 5 different fluorophores. MFA CXP Analysis Software. It is a system for the qualitative and quantitative measurement of the biological and physical properties of cells and particles. These properties are measured when the cells pass through one or two laser beams in an Indian file
- TRANSMISSION and SCANNING ELECTRON MICROSCOPY:
Philips Tecnai 20T analytical transmission Microscope: with EDAX microanalysis system and SIS digital camera. Transmission electron Microscope JEOL JEM 1010. Scanning electron Microscope XL 30 ESEM Philips. High resolution scanning electron microscope: Nova Nano SEM230 (FEG-SEM).
-ANIMAL HOUSING:
Establishment of animals and Integral Monitoring System for laboratory animals or CLAMS for food intake, food preferences, body weight, energy expenditure and indirect calorimetry, as well as for animals’ activity studies and biochemical parameters.
-LAFEMEX LABORATORY:
REDLabu Registry No. 400 (directed by Gema Medina-Gómez). Our vision is to have a space for murine phenotyping in vivo and ex vivo as well as molecular phenotyping, which would be aimed at the characterization of metabolic diseases of murine models. In short, this Laboratory is designed as a service to the scientific community.
Supervision, monitoring and analysis of measures of the comprehensive monitoring system for laboratory animals or CLAMS. Analysis of parameters measured by Bioplex. Study of both biochemical parameters and carbohydrate metabolism, through glucose and insulin tolerance tests.
SAN PABLO CEU UNIVERSITY
-INFRAESTRUCTURES:
qPCR, BioplexSystem, GC, HPLC, Luminometer, spectrophotometers, fluorimeter, plate reader, autoanalyzer, Cell culture unit.
-SERVICES:
Multiplex analysis of peptides and proteins in serum /plasma or tissue.
-OTHER SERVICES AVAILABLE IN THE FACULTY. METABOLOMIC AND BIOANALYSIS CENTER:
Experimental pharmacology; Metabolism and Body Composition Unit; X-ray diffraction and electron microscopy; Mass spectrometry; Nuclear magnetic resonance; Seahorse.
IIB ALBERTO SOLS
-SIERMAC:
High Field Magnetic Resonance Imaging and Spectroscopy Service.
-SERVICE OF COMPARATIVE MEDICINE:
Indirect calorimetry, Animal irradiator, Noninvasive Neurofunctional Evaluation (NINE), Behavior, In Vivo Image Analysis (IVIS).
- SERVICE OF CONFOCAL, TRANSMISSION and SCANNING ELECTRON MICROSCOPY
-GENOMIC SERVICE
-HISTOLOGY SERVICE
-FLOW CYTOMETRY SERVICE
- RADIO PROTECTION SERVICE
COMPLUTENSE UNIVERSITY (UCM)
The UCM has a series of Research Assistance Centers (CAI) that offer technical and scientific support to improve the infrastructure of researchers, facilitate the development of their Projects and offer advice. This is aimed at both UCM staff and other public and private entities. A list of all CAIs (ucm.es/cai) as well as their available equipment and services can be found on the UCM website. Below are those we consider closest to the scientific objectives of MOIR-2:
- CAI Bioimagen Complutense:
Nuclear Magnetic Resonance Unit, Brain Cartography Unit, Diagnostic Imaging Unit (X-ray and ultrasound for small animals, digitalization and image reading room, etc.), PET Preclinical Imaging Service
- CAI Biological Techniques:
Genomics Unit, Proteomics Unit, Flow Cytometry Unit and Fluorescence Microscopy.
- CAI Physical and Chemical Techniques:
X-ray Diffraction Unit, Mass Spectrometry Unit
- Research Support Workshops:
To design, build and modify parts and accessories of scientific devices. Review and repair of laboratory equipment.
- ANIMAL HOUSING
Breeding center, supply and use of experimental animals.
NATIONAL CARDIOVASCULAR RESEARCH CENTER CARLOS III (CNIC)
TECHNICAL UNITS
COMPARATIVE MEDICINE: 17200 cages for rodents, 1300 zebrafish tanks, 180 rabbit & 36 pig boxes. The large animal facilities contain a surgical theatre, dedicated cath labs, and a Langendorf system for pig hearts.
ADVANCED IMAGING: MRI, CT & PET, with organic chemistry and radiosynthesis labs for multimodal contrast agents/nanosystems & 2 platforms for intravital Microscopy in live rodents. Dedicated applications for small animals (high field 7T MR, nano PET/CT, ultrasound, IVIS, FMT), large animals (3T MRI, PET/CT, ultrasound & OCT), and humans (hybrid PET/MRI, 256-row CT scan & ultrasound).
GENOMICS: Equipped with 3 Illumina NGS instruments: HiSeq2500, GAIIx and MiSeq. Innovative research on single-cell genomics using a C1 Single-Cell Auto Prep microfluidic system from Fluidigm. 10Xgenomics
CELLOMICS: 3 last-generation flow cytometers; 3 high-speed sorters; automated magnetic sorter; liquid handling workstation; automated confocal microscope.
VIRAL VECTORS: Specialized in non-clinical grade recombinant viral vectors (lentivirus and AAV).
PROTEOMICS: Analysis, identification, quantification and characterization of proteins using latest generation mass spectrometers (QTRAP LC/MS/MS; Orbitrap XL ETD Hybrid Ion Trap-Orbitrap; Orbitrap Elite Hybrid Ion Trap-Orbitrap; Q Exactive Hybrid Quadrupole-Orbitrap; Orbitrap Fusion Quadrupole-Ion Trap-Orbitrap Tribrid), and 2 High- Performance Clusters HP Proliant DL980.
MICROSCOPY: Microscopes with multiphoton excitation sources and FLIM detectors (Zeiss 780, ISS-ALBA); Super-resolution super-continuum gSTED-3X and dSTORM; Multi-Color TIRF; 4 Multi-line Confocal State-of-Art microscopes; 4 Wide/bright field microscopes.
BIOINFORMATICS: Hi-throughput data analysis: 2 Hi-Performance Clusters (HPC), one with >1600 threads and 4.5TB RAM and a second HPC for the Galaxy Portal with 120 threads and 0.5TB RAM.
PLURIPOTENT CELL TECHNOLOGY: manipulation, genetic modification and differentiation of mouse and human pluripotent stem cells with 2 specialized culture rooms for human and mouse stem cells.
TRANSGENESIS: Production of genetically modified mice, with 2 state-of-the-art sets for microinjection; 4 stereomicroscopes (1 with fluorescence); laser ablation system; 2 microforges; electroporator; programmable bio-freezer; 4 CO2/O2 incubators, one for in vitro fertilization.